Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Identifieur interne : 000212 ( an2020/Analysis ); précédent : 000211; suivant : 000213Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Auteurs : Regina Konrad ; Ute Eberle [Allemagne] ; Alexandra Dangel ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
Affiliations:
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We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li></region><settlement><li>Munich</li></settlement></list><tree><noCountry><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name></noRegion><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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